Research > Team Non-Self Recognition in Fungi

Non-Self Recognition in Fungi

Principal Investigator : Sven Saupe



Team Members

Thierry Bardin - Doctorant MESR

Corinne Clavé - Professeur UB

Virginie Coustou-Linares - CRCN CNRS

Alexandra Granger Farbos - TCS UB

Frederique Ness - MCU UB

Mathieu Paoletti - CRCN CNRS

Sven Saupe - DR1 CNRS



Fungi are engaged in a variety of biotic interactions ranging from symbiosis to pathogenicity. We are interested in the mechanisms governing non-self recognition in this vast branch of the eukaryotic tree.


Research Activity

We study various aspects of the detection and response to non-self in fungi with a particular emphasis on a family of cytoplasmic receptors termed NLRs that control innate immunity and other biotic interaction processes in animal and plant lineages.



Programmed Cell death - Fungal immune response - Functional amyloid - NOD-like receptors - Pore forming protein –fungal-bacterial interactions


Selected publications 2009-2018

Tolchard J, Pandey MK, Berbon M, Noubhani A, Saupe SJ, Nishiyama Y, Habenstein B, Loquet A. Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR. J Biomol NMR. 2018 Mar;70(3):177-185

Deveau A, Bonito G, Uehling J, Paoletti M, Becker M, Bindschedler S, Hacquard S, Hervé V, Labbé J, Lastovetsky OA, Mieszkin S, Millet LJ, Vajna B, Junier P, Bonfante P, Krom BP, Olsson S, van Elsas JD, Wick LY. Bacterial-fungal interactions: ecology, mechanisms and challenges. FEMS Microbiol Rev. 2018 May 1;42(3):335-352

Heller J, Clavé C, Gladieux P, Saupe SJ, Glass NL. NLR surveillance of essential SEC-9 SNARE proteins induces programmed cell death upon allorecognition in filamentous fungi. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):E2292-E2301

Uehling J, Deveau A, Paoletti M. Do fungi have an innate immune response? An NLR-based comparison to plant and animal immune systems. PLoS Pathog. 2017 Oct;13(10):e1006578

Loquet A, Saupe SJ. Diversity of Amyloid Motifs in NLR Signaling in Fungi. Biomolecules. 2017 Apr 13;7(2)

Dheur S, Saupe SJ. (2016). Le vivant comme information. Une perspective subjective sur une crise en biologie. Revue des Sciences Sociales, n°56 « Indiscipliné.e.s. in press

Saupe SJ, Jarosz DF, True HL. Amyloid Prions in Fungi. Microbiol Spectr. 2016 Dec;4(6)

Stanek J, Andreas LB, Jaudzems K, Cala D, Lalli D, Bertarello A, Schubeis T, Akopjana I, Kotelovica S, Tars K, Pica A, Leone S, Picone D, Xu ZQ, Dixon NE, Martinez D, Berbon M, El Mammeri N, Noubhani A, Saupe S, Habenstein B, Loquet A, Pintacuda G. NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils. Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15504-15509

Riek R, Saupe SJ. The HET-S/s Prion Motif in the Control of Programmed Cell Death. Cold Spring Harb Perspect Biol. 2016 Sep 1;8(9)

Lamacchia M, Dyrka W, Breton A, Saupe SJ, Paoletti M. Overlapping Podospora anserina Transcriptional Responses to Bacterial and Fungal Non Self Indicate a Multilayered Innate Immune Response. Front Microbiol. 2016;7:471

Daskalov A, Habenstein B, Sabaté R, Berbon M, Martinez D, Chaignepain S, Coulary-Salin B, Hofmann K, Loquet A, Saupe SJ. Identification of a novel cell death-inducing domain reveals that fungal amyloid-controlled programmed cell death is related to necroptosis. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2720-5

Daskalov A, Dyrka W, Saupe SJ. Theme and variations: evolutionary diversification of the HET-s functional amyloid motif. Sci Rep. 2015 Jul 29;5:12494

Daskalov A, Saupe SJ. As a toxin dies a prion comes to life: A tentative natural history of the [Het-s] prion. Prion. 2015;9(3):184-9

Daskalov A, Habenstein B, Martinez D, Debets AJ, Sabaté R, Loquet A, Saupe SJ. Signal transduction by a fungal NOD-like receptor based on propagation of a prion amyloid fold. PLoS Biol. 2015 Feb;13(2):e1002059

Dyrka W, Lamacchia M, Durrens P, Kobe B, Daskalov A, Paoletti M, Sherman DJ, Saupe SJ. Diversity and variability of NOD-like receptors in fungi. Genome Biol Evol. 2014 Nov 13;6(12):3137-58

Daskalov A, Gantner M, Wälti MA, Schmidlin T, Chi CN, Wasmer C, Schütz A, Ceschin J, Clavé C, Cescau S, Meier B, Riek R, Saupe SJ. Contribution of specific residues of the ?-solenoid fold to HET-s prion function, amyloid structure and stability. PLoS Pathog. 2014 Jun;10(6):e1004158

Bastiaans E, Debets AJ, Aanen DK, van Diepeningen AD, Saupe SJ, Paoletti M. Natural variation of heterokaryon incompatibility gene het-c in Podospora anserina reveals diversifying selection. Mol Biol Evol. 2014 Apr;31(4):962-74

Bidard F, Clavé C, Saupe SJ. The transcriptional response to nonself in the fungus Podospora anserina. G3 (Bethesda). 2013 Jun 21;3(6):1015-30

Debets AJ, Dalstra HJ, Slakhorst M, Koopmanschap B, Hoekstra RF, Saupe SJ. High natural prevalence of a fungal prion. Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10432-7

Saupe SJ, Daskalov A. The [Het-s] prion, an amyloid fold as a cell death activation trigger. PLoS Pathog. 2012;8(5):e1002687

Daskalov A, Paoletti M, Ness F, Saupe SJ. Genomic clustering and homology between HET-S and the NWD2 STAND protein in various fungal genomes. PLoS One. 2012;7(4):e34854

Benkemoun L, Ness F, Sabaté R, Ceschin J, Breton A, Clavé C, Saupe SJ. Two structurally similar fungal prions efficiently cross-seed in vivo but form distinct polymers when coexpressed. Mol Microbiol. 2011 Dec;82(6):1392-405

Greenwald J, Buhtz C, Ritter C, Kwiatkowski W, Choe S, Maddelein ML, Ness F, Cescau S, Soragni A, Leitz D, Saupe SJ, Riek R. The mechanism of prion inhibition by HET-S. Mol Cell. 2010 Jun 25;38(6):889-99

Chevanne D, Saupe SJ, Clavé C, Paoletti M. WD-repeat instability and diversification of the Podospora anserina hnwd non-self recognition gene family. BMC Evol Biol. 2010 May 6;10:134

Paoletti M, Saupe SJ. Fungal incompatibility: evolutionary origin in pathogen defense? Bioessays. 2009 Nov;31(11):1201-10