Tél: 05 56 99 90 43
JP.Dirago (at) ibgc.u-bordeaux2.fr
Biologie moléculaire (PCR, Séquençage et Clonage d'ADN, Mutagenèse dirigée etc..); Transformation génétique des mitochondries (Biolistique); Electrophorèse (BN/CN-PAGE, SDS-PAGE); Western Blot; Production de protéines recombinantes; Purification de protéines; Oxygraphie; Microscopie électronique et à fluorescence...
Kucharczyk R, Ezkurdia N, Couplan E, Procaccio V, Ackerman SH, Blondel M, di Rago JP. Consequences of the pathogenic T9176C mutation of human mitochondrial DNA on yeast mitochondrial ATP synthase. Biochim Biophys Acta. 2010 June - July;1797(6-7):1105-1112
Sauvanet C, Duvezin-Caubet S, di Rago JP, Rojo M. Energetic requirements and bioenergetic modulation of mitochondrial morphology and dynamics. Semin Cell Dev Biol. 2010 Aug;21(6):558-65
Meulemans A, Seneca S, Pribyl T, Smet J, Alderweirldt V, Waeytens A, Lissens W, Van Coster R, De Meirleir L, di Rago JP, Gatti DL, Ackerman SH. Defining the pathogenesis of the human Atp12p W94R mutation using a Saccharomyces cerevisiae yeast model. J Biol Chem. 2010 Feb 5;285(6):4099-109
Kucharczyk R, Salin B, di Rago JP. Introducing the human Leigh syndrome mutation T9176G into Saccharomyces cerevisiae mitochondrial DNA leads to severe defects in the incorporation of Atp6p into the ATP synthase and in the mitochondrial morphology. Hum Mol Genet. 2009 Aug 1;18(15):2889-98
Ding MG, Butler CA, Saracco SA, Fox TD, Godard F, di Rago JP, Trumpower BL. Chapter 27 An improved method for introducing point mutations into the mitochondrial cytochrome B gene to facilitate studying the role of cytochrome B in the formation of reactive oxygen species. Methods Enzymol. 2009;456:491-506
Kucharczyk R, Rak M, di Rago JP. Biochemical consequences in yeast of the human mitochondrial DNA 8993T>C mutation in the ATPase6 gene found in NARP/MILS patients. Biochim Biophys Acta. 2009 May;1793(5):817-24
Ding MG, di Rago JP, Trumpower BL. Combining Inhibitor Resistance-conferring Mutations in Cytochrome b Creates Conditional Synthetic Lethality in Saccharomyces cerevisiae. J Biol Chem. 2009 Mar 27;284(13):8478-85
Kucharczyk R, Zick M, Bietenhader M, Rak M, Couplan E, Blondel M, Caubet SD, di Rago JP. Mitochondrial ATP synthase disorders: molecular mechanisms and the quest for curative therapeutic approaches. Biochim Biophys Acta. 2009 Jan;1793(1):186-99
Ding MG, Butler CA, Saracco SA, Fox TD, Godard F, di Rago JP, Trumpower BL. Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins. Biochim Biophys Acta. 2008 Sep;1777(9):1147-56
Goyon V, Fronzes R, Salin B, di-Rago JP, Velours J, Brèthes D. Yeast cells depleted in Atp14p fail to assemble Atp6p within the ATP synthase and exhibit altered mitochondrial cristae morphology. J Biol Chem. 2008 Apr 11;283(15):9749-58
Zeng X, Kucharczyk R, di Rago JP, Tzagoloff A. The leader peptide of yeast Atp6p is required for efficient interaction with the Atp9p ring of the mitochondrial ATPase. J Biol Chem. 2007 Dec 14;282(50):36167-76
Rak M, Tetaud E, Duvezin-Caubet S, Ezkurdia N, Bietenhader M, Rytka J, di Rago JP. A yeast model of the neurogenic ataxia retinitis pigmentosa (NARP) T8993G mutation in the mitochondrial ATP synthase-6 gene. J Biol Chem. 2007 Nov 23;282(47):34039-47
Rak M, Tetaud E, Godard F, Sagot I, Salin B, Duvezin-Caubet S, Slonimski PP, Rytka J, di Rago JP. Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology. J Biol Chem. 2007 Apr 13;282(15):10853-64
Ding MG, di Rago JP, Trumpower BL. Investigating the Qn site of the cytochrome bc1 complex in Saccharomyces cerevisiae with mutants resistant to ilicicolin H, a novel Qn site inhibitor. J Biol Chem. 2006 Nov 24;281(47):36036-43
Schwimmer C, Rak M, Lefebvre-Legendre L, Duvezin-Caubet S, Plane G, di Rago JP. Yeast models of human mitochondrial diseases: from molecular mechanisms to drug screening. Biotechnol J. 2006 Mar;1(3):270-81
Duvezin-Caubet S, Rak M, Lefebvre-Legendre L, Tetaud E, Bonnefoy N, di Rago JP. A "petite obligate" mutant of Saccharomyces cerevisiae: functional mtDNA is lethal in cells lacking the delta subunit of mitochondrial F1-ATPase. J Biol Chem. 2006 Jun 16;281(24):16305-13
Margeot A, Garcia M, Wang W, Tetaud E, di Rago JP, Jacq C. Why are many mRNAs translated to the vicinity of mitochondria: a role in protein complex assembly? Gene. 2005 Jul 18;354:64-71
Schwimmer C, Lefebvre-Legendre L, Rak M, Devin A, Slonimski PP, di Rago JP, Rigoulet M. Increasing mitochondrial substrate-level phosphorylation can rescue respiratory growth of an ATP synthase-deficient yeast. J Biol Chem. 2005 Sep 2;280(35):30751-9
Priault M, Salin B, Schaeffer J, Vallette FM, di Rago JP, Martinou JC. Impairing the bioenergetic status and the biogenesis of mitochondria triggers mitophagy in yeast. Cell Death Differ. 2005 Dec;12(12):1613-21
Lefebvre-Legendre L, Salin B, Schaëffer J, Brèthes D, Dautant A, Ackerman SH, di Rago JP. Failure to assemble the alpha 3 beta 3 subcomplex of the ATP synthase leads to accumulation of the alpha and beta subunits within inclusion bodies and the loss of mitochondrial cristae in Saccharomyces cerevisiae. J Biol Chem. 2005 May 6;280(18):18386-92
Duvezin-Caubet S, Caron M, Giraud MF, Velours J, di Rago JP. The two rotor components of yeast mitochondrial ATP synthase are mechanically coupled by subunit delta. Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13235-40
Lefebvre-Legendre L, Balguerie A, Duvezin-Caubet S, Giraud MF, Slonimski PP, Di Rago JP. F1-catalysed ATP hydrolysis is required for mitochondrial biogenesis in Saccharomyces cerevisiae growing under conditions where it cannot respire. Mol Microbiol. 2003 Mar;47(5):1329-39
Saint-Georges Y, Bonnefoy N, di Rago JP, Chiron S, Dujardin G. A pathogenic cytochrome b mutation reveals new interactions between subunits of the mitochondrial bc1 complex. J Biol Chem. 2002 Dec 20;277(51):49397-402
Giraud MF, Paumard P, Soubannier V, Vaillier J, Arselin G, Salin B, Schaeffer J, Brèthes D, di Rago JP, Velours J. Is there a relationship between the supramolecular organization of the mitochondrial ATP synthase and the formation of cristae? Biochim Biophys Acta. 2002 Sep 10;1555(1-3):174-80
Rempola B, Kaniak A, di Rago JP, Rytka J. Anaerobic growth of Saccharomyces cerevisiae alleviates the lethal effect of phosphotyrosyl phosphatase activators depletion. Acta Biochim Pol. 2001;48(4):1043-9
Paumard P, Vaillier J, Coulary B, Schaeffer J, Soubannier V, Mueller DM, Brèthes D, di Rago JP, Velours J. The ATP synthase is involved in generating mitochondrial cristae morphology. EMBO J. 2002 Feb 1;21(3):221-30
Brasseur G, Di Rago JP, Slonimski PP, Lemesle-Meunier D. Analysis of suppressor mutation reveals long distance interactions in the bc(1) complex of Saccharomyces cerevisiae. Biochim Biophys Acta. 2001 Aug 17;1506(2):89-102
Lefebvre-Legendre L, Vaillier J, Benabdelhak H, Velours J, Slonimski PP, di Rago JP. Identification of a nuclear gene (FMC1) required for the assembly/stability of yeast mitochondrial F(1)-ATPase in heat stress conditions. J Biol Chem. 2001 Mar 2;276(9):6789-96
Rempola B, Kaniak A, Migdalski A, Rytka J, Slonimski PP, di Rago JP. Functional analysis of RRD1 (YIL153w) and RRD2 (YPL152w), which encode two putative activators of the phosphotyrosyl phosphatase activity of PP2A in Saccharomyces cerevisiae. Mol Gen Genet. 2000 Jan;262(6):1081-92
di Rago J.P., Bonnefoy N. and Duvezin-Caubet S. (2004) Industrial method for producing RNA and system for carrying out said method. N°10/564,512, du 22/07/2004.
di Rago J.P., Rak M., Tetaud E., Duvezin-Caubet S. et Kucharczyk R. (2006) Modélisation chez la levure des mutations du gène mitochondrial ATP6 responsables du syndrome NARP chez l'homme et ses applications pour le criblage de médicaments. N°06/03934, du 03/05/2006.
